› 1tri

X-ray diffraction
2.4Å resolution

THE CRYSTAL STRUCTURE OF AN ENGINEERED MONOMERIC TRIOSEPHOSPHATE ISOMERASE, MONOTIM: THE CORRECT MODELLING OF AN EIGHT-RESIDUE LOOP

Released:
DOI: 10.2210/pdb1tri/pdb
PDB entry 1tri coloured by chain, front view.
PDB entry 1tri coloured by chain, side view.
PDB entry 1tri coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
The crystal structure of an engineered monomeric triosephosphate isomerase, monoTIM: the correct modelling of an eight-residue loop.
Borchert TV, Abagyan R, Kishan KV, Zeelen JP, Wierenga RK
Structure 1 205-13 (1993)
PMID: 16100954

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138175 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase, glycosomal Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 26.09 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Not provided
UniProt:
  • Canonical: P04789 (Residues: 1-250; Coverage: 97%)
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 83.78Å b: 42.8Å c: 68.84Å
α: 90° β: 108.22° γ: 90°
R-values:
R R work R free
0.165 0.165 not available
Expression system: Not provided

Quick links

Citations

6 review citations

The TIM-barrel fold: a versatile framework for efficient enzymes.
Wierenga RK. (2001)
5 more

1 mention without citation

Reflections on the catalytic power of a TIM-barrel.
Richard et al. (2014)