› 1tf0

X-ray diffraction
2.7Å resolution

Crystal structure of the GA module complexed with human serum albumin

Released:
DOI: 10.2210/pdb1tf0/pdb
PDB entry 1tf0 coloured by chain, front view.
PDB entry 1tf0 coloured by chain, side view.
PDB entry 1tf0 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure and biological implications of a bacterial albumin binding module in complex with human serum albumin.
Lejon S, Frick IM, Björck L, Wikström M, Svensson S
J Biol Chem 279 42924-8 (2004)
PMID: 15269208

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136183 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Albumin Chain: A
Molecule details ›
Chain: A
Length: 572 amino acids
Theoretical weight: 65.32 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P02768 (Residues: 25-596; Coverage: 97%)
Gene names: ALB, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341
Sequence domains: Serum albumin family
Structure domains: Serum Albumin; Chain A, Domain 1
Peptostreptococcal albumin-binding protein Chain: B
Molecule details ›
Chain: B
Length: 53 amino acids
Theoretical weight: 5.94 KDa
Source organism: Finegoldia magna ATCC 29328
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51911 (Residues: 213-265; Coverage: 15%)
Gene name: pab
Sequence domains: GA-like domain
Structure domains: Albumin-binding domain

Ligands and Environments

2 bound ligands:
Ligand DKA 3 x DKA
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 96.295Å b: 134.799Å c: 122.463Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.249 0.295
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Albumin: pathophysiologic basis of its role in the treatment of cirrhosis and its complications.
Garcia-Martinez et al. (2013)
12 more

10 mentions without citation

Non-covalent albumin-binding ligands for extending the circulating half-life of small biotherapeutics.
Zorzi et al. (2019)
9 more