› 1tdh

X-ray diffraction
2.1Å resolution

Crystal structure of human endonuclease VIII-like 1 (NEIL1)

Released:
DOI: 10.2210/pdb1tdh/pdb
PDB entry 1tdh coloured by chain, front view.
PDB entry 1tdh coloured by chain, side view.
PDB entry 1tdh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human endonuclease VIII-like 1 (NEIL1) reveals a zincless finger motif required for glycosylase activity.
Doublié S, Bandaru V, Bond JP, Wallace SS
Proc Natl Acad Sci U S A 101 10284-9 (2004)
PMID: 15232006

Function and Biology Details

Reaction catalysed: 
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease 8-like 1 Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 40.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96FI4 (Residues: 1-390; Coverage: 93%)
Gene name: NEIL1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: R3
Unit cell:
a: 132.19Å b: 132.19Å c: 51.09Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.194 0.231
Expression system: Escherichia coli

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Citations

24 review citations

Base-excision repair of oxidative DNA damage.
David et al. (2007)
23 more

22 mentions without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)
21 more