› 1tcb

X-ray diffraction
2.1Å resolution

THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA

Released:
DOI: 10.2210/pdb1tcb/pdb
PDB entry 1tcb coloured by chain, front view.
PDB entry 1tcb coloured by chain, side view.
PDB entry 1tcb coloured by chain, top view.
Source organism: Moesziomyces antarcticus
Primary publication:
The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica.
Uppenberg J, Hansen MT, Patkar S, Jones TA
Structure 2 293-308 (1994)
PMID: 8087556

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154386 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lipase B Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 33.04 KDa
Source organism: Moesziomyces antarcticus
Expression system: Not provided
UniProt:
  • Canonical: P41365 (Residues: 26-342; Coverage: 98%)
Structure domains: alpha/beta hydrolase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand BOG 2 x BOG
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 69.2Å b: 50.5Å c: 86.7Å
α: 90° β: 101.5° γ: 90°
R-values:
R R work R free
0.187 0.187 not available
Expression system: Not provided

Quick links

Citations

19 review citations

Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases.
Jaeger et al. (1999)
18 more

7 mentions without citation

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
6 more