› 1t9h

X-ray diffraction
1.6Å resolution

The crystal structure of YloQ, a circularly permuted GTPase.

Released:
DOI: 10.2210/pdb1t9h/pdb
PDB entry 1t9h coloured by chain, front view.
PDB entry 1t9h coloured by chain, side view.
PDB entry 1t9h coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The crystal structure of YloQ, a circularly permuted GTPase essential for Bacillus subtilis viability.
Levdikov VM, Blagova EV, Brannigan JA, Cladière L, Antson AA, Isupov MN, Séror SJ, Wilkinson AJ
J Mol Biol 340 767-82 (2004)
PMID: 15223319

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128556 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small ribosomal subunit biogenesis GTPase RsgA Chain: A
Molecule details ›
Chain: A
Length: 307 amino acids
Theoretical weight: 35.03 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O34530 (Residues: 1-298; Coverage: 100%)
Gene names: BSU15780, cpgA, engC, rsgA, yloQ
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand IUM 8 x IUM
Ligand ZN 1 x ZN
Ligand CA 4 x CA
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P43212
Unit cell:
a: 47.97Å b: 47.97Å c: 279.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.146 0.144 0.178
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structure-function relationships of the G domain, a canonical switch motif.
Wittinghofer et al. (2011)
7 more

5 mentions without citation

Structural stabilization of GTP-binding domains in circularly permuted GTPases: implications for RNA binding.
Anand et al. (2006)
4 more