› 1t8k

X-ray diffraction
1.1Å resolution

Crystal Structure of apo acyl carrier protein from E. coli

Released:
DOI: 10.2210/pdb1t8k/pdb
PDB entry 1t8k coloured by chain, front view.
PDB entry 1t8k coloured by chain, side view.
PDB entry 1t8k coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions.
Qiu X, Janson CA
Acta Crystallogr D Biol Crystallogr 60 1545-54 (2004)
PMID: 15333924

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141273 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl carrier protein Chain: A
Molecule details ›
Chain: A
Length: 77 amino acids
Theoretical weight: 8.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6A8 (Residues: 2-78; Coverage: 99%)
Gene names: JW1080, acpP, b1094
Sequence domains: Phosphopantetheine attachment site
Structure domains: ACP-like

Ligands and Environments

2 bound ligands:
Ligand ZN 10 x ZN
Ligand IMD 6 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 27.51Å b: 41.26Å c: 62.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.133 0.15
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The ubiquitous carrier protein--a window to metabolite biosynthesis.
Mercer et al. (2007)
3 more

25 mentions without citation

Fatty acid biosynthesis revisited: structure elucidation and metabolic engineering.
Beld et al. (2015)
24 more