› 1t87

X-ray diffraction
1.8Å resolution

Crystal Structure of the Ferrous CO-bound Cytochrome P450cam (C334A)

Released:
DOI: 10.2210/pdb1t87/pdb
PDB entry 1t87 coloured by chain, front view.
PDB entry 1t87 coloured by chain, side view.
PDB entry 1t87 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Crystal structure of the cytochrome p450cam mutant that exhibits the same spectral perturbations induced by putidaredoxin binding.
Nagano S, Tosha T, Ishimori K, Morishima I, Poulos TL
J Biol Chem 279 42844-9 (2004)
PMID: 15269210

Function and Biology Details

Reaction catalysed: 
(+)-camphor + reduced putidaredoxin + O(2) = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132314 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Camphor 5-monooxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 414 amino acids
Theoretical weight: 46.56 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00183 (Residues: 2-415; Coverage: 100%)
Gene names: camC, cyp101
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
4 bound ligands:
Ligand K 3 x K
Ligand CAM 2 x CAM
Ligand CMO 1 x CMO
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 67.03Å b: 62.23Å c: 94.92Å
α: 90° β: 90.48° γ: 90°
R-values:
R R work R free
0.185 0.185 0.207
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Heme enzyme structure and function.
Poulos TL. (2014)
10 more

6 mentions without citation

Protein dynamics in cytochrome P450 molecular recognition and substrate specificity using 2D IR vibrational echo spectroscopy.
Thielges et al. (2011)
5 more