› 1t0f

X-ray diffraction
1.85Å resolution

Crystal Structure of the TnsA/TnsC(504-555) complex

Released:
DOI: 10.2210/pdb1t0f/pdb
PDB entry 1t0f coloured by chain, front view.
PDB entry 1t0f coloured by chain, side view.
PDB entry 1t0f coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The carboxy-terminal portion of TnsC activates the Tn7 transposase through a specific interaction with TnsA.
Ronning DR, Li Y, Perez ZN, Ross PD, Hickman AB, Craig NL, Dyda F
EMBO J 23 2972-81 (2004)
PMID: 15257292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-138797 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transposon Tn7 transposition protein TnsA Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 31.51 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13988 (Residues: 1-273; Coverage: 100%)
Gene name: tnsA
Sequence domains:
Structure domains:
Transposon Tn7 transposition protein TnsC Chains: C, D
Molecule details ›
Chains: C, D
Length: 54 amino acids
Theoretical weight: 6.1 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05846 (Residues: 503-555; Coverage: 10%)
Gene name: tnsC
Sequence domains: Tn7 transposition regulator TnsC
Structure domains: Arc Repressor Mutant, subunit A

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand MLA 3 x MLA
Ligand MPD 5 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 156.032Å b: 70.112Å c: 89.681Å
α: 90° β: 123.61° γ: 90°
R-values:
R R work R free
0.211 0.211 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Integrating prokaryotes and eukaryotes: DNA transposases in light of structure.
Hickman et al. (2010)
2 more

4 mentions without citation

The PD-(D/E)XK superfamily revisited: identification of new members among proteins involved in DNA metabolism and functional predictions for domains of (hitherto) unknown function.
Kosinski et al. (2005)
3 more