› 1szr

X-ray diffraction
2.15Å resolution

A Dimer interface mutant of ornithine decarboxylase reveals structure of gem diamine intermediate

Released:
DOI: 10.2210/pdb1szr/pdb
PDB entry 1szr coloured by chain, front view.
PDB entry 1szr coloured by chain, side view.
PDB entry 1szr coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Multiple active site conformations revealed by distant site mutation in ornithine decarboxylase.
Jackson LK, Baldwin J, Akella R, Goldsmith EJ, Phillips MA
Biochemistry 43 12990-9 (2004)
PMID: 15476392

Function and Biology Details

Reaction catalysed: 
L-ornithine = putrescine + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139767 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ornithine decarboxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 425 amino acids
Theoretical weight: 47 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P07805 (Residues: 1-423; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PXP 2 x PXP
2 bound ligands:
Ligand PLG 1 x PLG
Ligand ORX 1 x ORX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 67.468Å b: 152.444Å c: 155.007Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.279
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Nutritional and medicinal aspects of D-amino acids.
Friedman et al. (2012)
1 more

2 mentions without citation

Polyamines in protozoan pathogens.
Phillips MA. (2018)
1 more