1sx8 Summary


EcoRV bound to cognate DNA and Mn2+

The structure was published by Horton, N.C. and Perona, J.J., in 2004 in a paper entitled "DNA Cleavage by EcoRV Endonuclease: Two Metal Ions in Three Metal Ion Binding Sites" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.15 Å and deposited in 2004.

The experimental data on which the structure is based was not deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely 5'-D(*C*AP*AP*GP*AP*TP*AP*TP*CP*TP*T)-3' and Type II restriction enzyme EcoRV.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterotetramers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Type II restriction enzyme EcoRV P04390 (2-245) (T2E5_ECOLX)search Escherichia colisearch 100% 244 98%
B Type II restriction enzyme EcoRV P04390 (2-245) (T2E5_ECOLX)search Escherichia colisearch 100% 244 98%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P04390 (2 - 245) Type II restriction enzyme EcoRV Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B (P04390) Restriction endonuclease EcoRVsearch ECO RV Endonuclease; Chain Asearch PF09233: Restriction endonuclease EcoRVsearch

Chain ID Molecular function (GO) Biological process (GO)
A, B (P04390) endonuclease activitysearch nuclease activitysearch DNA bindingsearch Type II site-specific deoxyribonuclease activitysearch hydrolase activitysearch metal ion bindingsearch nucleic acid phosphodiester bond hydrolysissearch DNA catabolic process, endonucleolyticsearch DNA restriction-modification systemsearch

Chain InterPro annotation
A, B Restriction endonuclease type II-likesearch DNA mismatch repair MutH/Restriction endonuclease, type IIsearch Restriction endonuclease, type II, EcoRVsearch