› 1sws

X-ray diffraction
2Å resolution

CORE-STREPTAVIDIN MUTANT D128A AT PH 4.5

Released:
DOI: 10.2210/pdb1sws/pdb
PDB entry 1sws coloured by chain, front view.
PDB entry 1sws coloured by chain, side view.
PDB entry 1sws coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
A structural snapshot of an intermediate on the streptavidin-biotin dissociation pathway.
Freitag S, Chu V, Penzotti JE, Klumb LA, To R, Hyre D, Le Trong I, Lybrand TP, Stenkamp RE, Stayton PS
Proc Natl Acad Sci U S A 96 8384-9 (1999)
PMID: 10411884

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 127 amino acids
Theoretical weight: 13.24 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 37-163; Coverage: 80%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 48Å b: 66.3Å c: 82.3Å
α: 90° β: 97.3° γ: 90°
R-values:
R R work R free
0.191 not available 0.272
Expression system: Escherichia coli

Quick links

Citations

30 citation in other articles

Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity.
Boder et al. (2000)
29 more

2 mentions without citation

Improved affinity of engineered streptavidin for the Strep-tag II peptide is due to a fixed open conformation of the lid-like loop at the binding site.
Korndörfer et al. (2002)
1 more