› 1sll

X-ray diffraction
2Å resolution

SIALIDASE L FROM LEECH MACROBDELLA DECORA

Released:
DOI: 10.2210/pdb1sll/pdb
PDB entry 1sll coloured by chain, front view.
PDB entry 1sll coloured by chain, side view.
PDB entry 1sll coloured by chain, top view.
Source organism: Macrobdella decora
Primary publication:
The crystal structure of an intramolecular trans-sialidase with a NeuAc alpha2-->3Gal specificity.
Luo Y, Li SC, Chou MY, Li YT, Luo M
Structure 6 521-30 (1998)
PMID: 9562562

Function and Biology Details

Reaction catalysed: 
Elimination of alpha-sialyl groups in N-acetylneuraminic acid glycosides, releasing 2,7-anhydro-alpha-N-acetylneuraminate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173277 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Anhydrosialidase Chain: A
Molecule details ›
Chain: A
Length: 679 amino acids
Theoretical weight: 74.57 KDa
Source organism: Macrobdella decora
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q27701 (Residues: 81-759; Coverage: 92%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P1
Unit cell:
a: 46.364Å b: 69.275Å c: 72.469Å
α: 113.48° β: 95.41° γ: 107.31°
R-values:
R R work R free
0.167 0.167 0.224
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Lectins.
Vijayan et al. (1999)
2 more

2 mentions without citation

Unraveling virus relationships by structure-based phylogenetic classification.
Ng et al. (2020)
1 more