› 1skq

X-ray diffraction
1.8Å resolution

The crystal structure of Sulfolobus solfataricus elongation factor 1-alpha in complex with magnesium and GDP

Released:
DOI: 10.2210/pdb1skq/pdb
PDB entry 1skq coloured by chain, front view.
PDB entry 1skq coloured by chain, side view.
PDB entry 1skq coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
The crystal structure of Sulfolobus solfataricus elongation factor 1alpha in complex with magnesium and GDP.
Vitagliano L, Ruggiero A, Masullo M, Cantiello P, Arcari P, Zagari A
Biochemistry 43 6630-6 (2004)
PMID: 15157096

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152768 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Elongation factor 1-alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 435 amino acids
Theoretical weight: 48.54 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35021 (Residues: 1-435; Coverage: 100%)
Gene names: SSO0216, tef1, tuf
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 61.95Å b: 113.83Å c: 81.08Å
α: 90° β: 90.1° γ: 90°
R-values:
R R work R free
0.23 0.219 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Structural models of human eEF1A1 and eEF1A2 reveal two distinct surface clusters of sequence variation and potential differences in phosphorylation.
Soares et al. (2009)
5 more

12 mentions without citation

Review: Translational GTPases.
Maracci et al. (2016)
11 more