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EBI PDBe PDBeView

PDBe Entry: 1skm view

HhaI methyltransferase in complex with DNA containing an abasic south carbocyclic sugar at its target site
Summary
Header TRANSFERASE/DNAsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 2.2 Å, R-factor: 18.3%, Free R-factor: 22.1%, Spacegroup: H 3 2
Released 24/08/2004, deposition: 05/03/2004, last revision: 24/02/2009
Authors Horton, J.R.search; Ratner, G.search; Banavali, N.search; Huang, N.search; Marquez, V.E.search; MacKerell, A.D.search; Cheng, X.search
Primary citation Caught in the act: visualization of an intermediate in the DNA base-flipping pathway induced by HhaI methyltransferase
NUCLEIC ACIDS RES.search vol:32, pag:3877-3886 (2004) [PubMed ID 15273274 ]search
Keywords PROTEIN-DNA COMPLEX Containing Constrained Abasic Unnatural basesearch, TRANSFERASE/DNA COMPLEXsearch
EC 2.1.1.37 ExPASy BRENDA search (A)
Organism Haemophilus haemolyticus 726search(A)
UniProt Modification methylase HhaI (EC 2.1.1.37) (M.HhaI) (Cytosine-specific methyltransferase HhaI) P05102search (A)
Solvent A, C, D
Related entries 1hmy, 1mht, 1fjx, 9mht
Polymers
Id Name Type UniProt Residues Observed
C 5'-D(*TP*CP*CP*AP*TP*GP*CP*GP*CP*TP*GP*AP*C)-3' DNA
13 100%
D 5'-D(*T*GP*TP*CP*AP*GP*(HCX)P*GP*CP*AP*TP*GP*G)-3' DNA
13 92%
A Modification methylase HhaI Protein P05102 (MTH1_HAEPH)search
 327 100%
Heterogens
Id Name Ligands
A S-ADENOSYL-L-HOMOCYSTEINE SAH search
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