1sk6 Summary


Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF) in complex with calmodulin, 3',5' cyclic AMP (cAMP), and pyrophosphate

The structure was published by Guo, Q., Shen, Y., Zhukovskaya, N.L., Florian, J., and Tang, W.J., in 2004 in a paper entitled "Structural and kinetic analyses of the interaction of anthrax adenylyl cyclase toxin with reaction products cAMP and pyrophosphate." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.2 Å and deposited in 2004.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Calmodulin-sensitive adenylate cyclase and Calmodulin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Calmodulin-sensitive adenylate cyclase P40136 (291-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 510 94%
B Calmodulin-sensitive adenylate cyclase P40136 (291-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 510 94%
C Calmodulin-sensitive adenylate cyclase P40136 (291-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 510 94%
D Calmodulin P62158 (2-149) (CALM_HUMAN)search Homo sapienssearch 97% 148 96%
E Calmodulin P62158 (2-149) (CALM_HUMAN)search Homo sapienssearch 97% 148 96%
F Calmodulin P62158 (2-149) (CALM_HUMAN)search Homo sapienssearch 97% 148 96%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P40136 (291 - 800) Calmodulin-sensitive adenylate cyclase Bacillus anthracis
P62158 (2 - 149) Calmodulin Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C (P40136) Adenylylcyclase toxin (the edema factor)search PF03497: Anthrax toxin LF subunitsearch
D, E, F (P62158) Calmodulin-likesearch EF-handsearch PF13499: EF-hand domain pairsearch, PF13833: EF-hand domain pairsearch

Chain ID Molecular function (GO) Cellular component (GO) Biological process (GO)
A, B, C (P40136) calcium- and calmodulin-responsive adenylate cyclase activitysearch extracellular regionsearch pathogenesissearch
D, E, F (P62158) calcium ion bindingsearch phosphatidylinositol 3-kinase bindingsearch protein bindingsearch protein N-terminus bindingsearch ion channel bindingsearch metal ion bindingsearch adenylate cyclase bindingsearch calcium-dependent protein bindingsearch protein domain specific bindingsearch enzyme regulator activitysearch titin bindingsearch phospholipase bindingsearch protein phosphatase activator activitysearch nitric-oxide synthase regulator activitysearch type 3 metabotropic glutamate receptor bindingsearch nitric-oxide synthase bindingsearch thioesterase bindingsearch N-terminal myristoylation domain bindingsearch cytosolsearch plasma membranesearch nucleoplasmsearch spindlesearch extracellular regionsearch cytoskeletonsearch neuron projectionsearch centrosomesearch nucleussearch extracellular vesicular exosomesearch cytoplasmsearch growth conesearch spindle microtubulesearch calcium channel complexsearch spindle polesearch sarcomeresearch glucose metabolic processsearch regulation of high voltage-gated calcium channel activitysearch regulation of nitric-oxide synthase activitysearch regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulumsearch positive regulation of cyclic nucleotide metabolic processsearch regulation of cell communication by electrical coupling involved in cardiac conductionsearch detection of calcium ionsearch positive regulation of nitric-oxide synthase activitysearch regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ionsearch rhodopsin mediated signaling pathwaysearch epidermal growth factor receptor signaling pathwaysearch small molecule metabolic processsearch calcium-mediated signalingsearch platelet degranulationsearch platelet activationsearch membrane organizationsearch substantia nigra developmentsearch regulation of heart ratesearch signal transductionsearch positive regulation of phosphoprotein phosphatase activitysearch phototransduction, visible lightsearch response to corticosteronesearch muscle contractionsearch innate immune responsesearch positive regulation of cyclic-nucleotide phosphodiesterase activitysearch fibroblast growth factor receptor signaling pathwaysearch neurotrophin TRK receptor signaling pathwaysearch glycogen catabolic processsearch activation of adenylate cyclase activitysearch inositol phosphate metabolic processsearch nitric oxide metabolic processsearch negative regulation of ryanodine-sensitive calcium-release channel activitysearch positive regulation of ryanodine-sensitive calcium-release channel activitysearch regulation of cytokinesissearch regulation of rhodopsin mediated signaling pathwaysearch regulation of ryanodine-sensitive calcium-release channel activitysearch blood coagulationsearch carbohydrate metabolic processsearch synaptic transmissionsearch response to amphetaminesearch G-protein coupled receptor signaling pathwaysearch activation of phospholipase C activitysearch positive regulation of protein dephosphorylationsearch response to calcium ionsearch Fc-epsilon receptor signaling pathwaysearch regulation of cardiac muscle contractionsearch

Chain InterPro annotation
A, B, C Anthrax toxin, edema factor, centralsearch
D, E, F EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch