› 1sfx

X-ray diffraction
1.55Å resolution

X-ray crystal structure of putative HTH transcription regulator from Archaeoglobus fulgidus

Released:
DOI: 10.2210/pdb1sfx/pdb
PDB entry 1sfx coloured by chain, front view.
PDB entry 1sfx coloured by chain, side view.
PDB entry 1sfx coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Entry authors: Osipiuk J, Skarina T, Savchenko A, Edwards A, Cymborowski M, Minor W, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-128000 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription regulator TrmB N-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 109 amino acids
Theoretical weight: 12.78 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O28271 (Residues: 1-106; Coverage: 100%)
Gene name: AF_2008
Sequence domains: Sugar-specific transcriptional regulator TrmB
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand EDO 3 x EDO
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 64.265Å b: 59.634Å c: 63.498Å
α: 90° β: 90.11° γ: 90°
R-values:
R R work R free
0.15 0.148 0.183
Expression system: Escherichia coli

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