› 1sft

X-ray diffraction
1.9Å resolution

ALANINE RACEMASE

Released:
DOI: 10.2210/pdb1sft/pdb
PDB entry 1sft coloured by chain, front view.
PDB entry 1sft coloured by chain, side view.
PDB entry 1sft coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Determination of the structure of alanine racemase from Bacillus stearothermophilus at 1.9-A resolution.
Shaw JP, Petsko GA, Ringe D
Biochemistry 36 1329-42 (1997)
PMID: 9063881

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.57 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10724 (Residues: 1-388; Coverage: 100%)
Gene names: alr, dal
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 98.59Å b: 90.13Å c: 85.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.267
Expression system: Escherichia coli

Quick links

Citations

23 review citations

Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations.
Eliot et al. (2004)
22 more

16 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
15 more