› 1sfr

X-ray diffraction
2.7Å resolution

Crystal Structure of the Mycobacterium tuberculosis Antigen 85A Protein

Released:
DOI: 10.2210/pdb1sfr/pdb
PDB entry 1sfr coloured by chain, front view.
PDB entry 1sfr coloured by chain, side view.
PDB entry 1sfr coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Mycobacterium tuberculosis antigen 85A and 85C structures confirm binding orientation and conserved substrate specificity.
Ronning DR, Vissa V, Besra GS, Belisle JT, Sacchettini JC
J Biol Chem 279 36771-7 (2004)
PMID: 15192106

Function and Biology Details

Reactions catalysed: 
2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate
Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-162090 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diacylglycerol acyltransferase/mycolyltransferase Ag85A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 304 amino acids
Theoretical weight: 32.74 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WQP3 (Residues: 43-338; Coverage: 100%)
Gene names: MTV026.09c, Rv3804c, fbpA, mpt44
Sequence domains: Putative esterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2221
Unit cell:
a: 131.43Å b: 288.662Å c: 101.289Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.222 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Antituberculosis drugs: ten years of research.
Janin YL. (2007)
7 more

10 mentions without citation

A structural overview of mycobacterial adhesins: Key biomarkers for diagnostics and therapeutics.
Squeglia et al. (2018)
9 more