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EBI PDBe PDBeView

PDBe Entry: 1seu view

Human DNA Topoisomerase I (70 Kda) In Complex With The Indolocarbazole SA315F and Covalent Complex With A 22 Base Pair DNA Duplex
Summary
Header ISOMERASE/DNAsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 3.0 Å, R-factor: 23.7%, Free R-factor: 29.5%, Spacegroup: P 1 21 1
Released 19/04/2005, deposition: 18/02/2004, last revision: 24/02/2009
Authors Staker, B.L.search; Feese, M.D.search; Cushman, M.search; Pommier, Y.search; Zembower, D.search; Stewart, L.search; Burgin, A.B.search
Primary citation Structures of three classes of anticancer agents bound to the human topoisomerase I-DNA covalent complex
J.MED.CHEM.search vol:48, pag:2336-2345 (2005) [PubMed ID 15801827 ]search
Keywords COMPLEX (ISOMERASE/DNA)search, DNAsearch, TOPOISOMERASE Isearch, DRUGsearch, POISONsearch
EC 5.99.1.2 ExPASy BRENDA search (A)
Organism Homo sapiens(human) 9606search(A)
UniProt DNA topoisomerase 1 (EC 5.99.1.2) (DNA topoisomerase I) P11387search (A)
Related entries 1sc7, 1k4t
Polymers
Id Name Type UniProt Residues Observed
B 5'-D(*AP*AP*AP*AP*AP*GP*AP*CP*TP*T)-3' DNA
10 100%
C 5'-D(*(TGP)P*GP*AP*AP*AP*AP*AP*TP*TP*TP*TP*T)-3' DNA
12 100%
D 5'-D(*AP*AP*AP*AP*AP*TP*TP*TP*TP*TP*CP*CP*AP*AP*GP*TP*CP*TP*TP*TP*TP*T)-3' DNA
22 100%
A DNA topoisomerase I Protein P11387 (TOP1_HUMAN)search
 592 95%
Heterogens
Id Name Ligands
D 2,10-DIHYDROXY-12-(BETA-D-GLUCOPYRANOSYL)-6,7,12,13-TETRAHYDROINDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOLE-5,7-DIONE SA3 search
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