1sa3 Summary


An asymmetric complex of restriction endonuclease MspI on its palindromic DNA recognition site

The structure was published by Xu, Q.S., Kucera, R.B., Roberts, R.J., and Guo, H.C., in 2004 in a paper entitled "An Asymmetric Complex of Restriction Endonuclease MspI on Its Palindromic DNA Recognition Site." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.95 Å and deposited in 2004.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely 5'-D(*CP*CP*CP*CP*CP*GP*GP*GP*GP*G)-3' and Type II restriction enzyme MspI.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Type II restriction enzyme MspI P11405 (1-262) (T2M1_MORSP)search Moraxella sp.search 100% 262 100%
B Type II restriction enzyme MspI P11405 (1-262) (T2M1_MORSP)search Moraxella sp.search 100% 262 100%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P11405 (1 - 262) Type II restriction enzyme MspI Moraxella sp.

Chain Structural classification (SCOP) Sequence family (Pfam)
A, B (P11405) Restriction endonuclease MspIsearch PF09208: Restriction endonuclease MspIsearch

Chain ID Biological process (GO) Molecular function (GO)
A, B (P11405) DNA catabolic process, endonucleolyticsearch nucleic acid phosphodiester bond hydrolysissearch DNA restriction-modification systemsearch nuclease activitysearch Type II site-specific deoxyribonuclease activitysearch hydrolase activitysearch endonuclease activitysearch

Chain InterPro annotation
A, B Restriction endonuclease type II-likesearch Restriction endonuclease, type II, MspIsearch