› 1s6l

Solution NMR

Solution structure of MerB, the Organomercurial Lyase involved in the bacterial mercury resistance system

Released:
DOI: 10.2210/pdb1s6l/pdb
PDB entry 1s6l coloured by chain, ensemble of 20 models, front view.
PDB entry 1s6l coloured by chain, ensemble of 20 models, side view.
PDB entry 1s6l coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli
Primary publication:
NMR structural studies reveal a novel protein fold for MerB, the organomercurial lyase involved in the bacterial mercury resistance system.
Di Lello P, Benison GC, Valafar H, Pitts KE, Summers AO, Legault P, Omichinski JG
Biochemistry 43 8322-32 (2004)
PMID: 15222745

Function and Biology Details

Reaction catalysed: 
An alkylmercury + H(+) = an alkane + Hg(2+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160120 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkylmercury lyase Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77072 (Residues: 1-212; Coverage: 100%)
Gene name: merB
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: Simulated annealing, with a combination of torsion angle dynamics and cartesian dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

A bacterial view of the periodic table: genes and proteins for toxic inorganic ions.
Silver et al. (2005)
2 more