› 1s56

X-ray diffraction
2.43Å resolution

Crystal Structure of "Truncated" Hemoglobin N (HbN) from Mycobacterium tuberculosis, Soaked with Xe Atoms

Released:
DOI: 10.2210/pdb1s56/pdb
PDB entry 1s56 coloured by chain, front view.
PDB entry 1s56 coloured by chain, side view.
PDB entry 1s56 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Heme-ligand tunneling in group I truncated hemoglobins.
Milani M, Pesce A, Ouellet Y, Dewilde S, Friedman J, Ascenzi P, Guertin M, Bolognesi M
J Biol Chem 279 21520-5 (2004)
PMID: 15016811

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161826 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Group 1 truncated hemoglobin GlbN Chains: A, B
Molecule details ›
Chains: A, B
Length: 136 amino acids
Theoretical weight: 14.46 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WN25 (Residues: 1-136; Coverage: 100%)
Gene names: MTCY48.23, Rv1542c, glbN
Sequence domains: Bacterial-like globin
Structure domains: Globins

Ligands and Environments

6 bound ligands:
Ligand CYN 2 x CYN
Ligand K 1 x K
Ligand HEC 1 x HEC
Ligand XE 8 x XE
Ligand PO4 1 x PO4
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 44.321Å b: 61.959Å c: 90.747Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.194 0.277
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structure and regulation of soluble guanylate cyclase.
Derbyshire et al. (2012)
7 more

5 mentions without citation

Nitrosylation mechanisms of Mycobacterium tuberculosis and Campylobacter jejuni truncated hemoglobins N, O, and P.
Ascenzi et al. (2014)
4 more