› 1s3l

X-ray diffraction
2.4Å resolution

Structural and Functional Characterization of a Novel Archaeal Phosphodiesterase

Released:
DOI: 10.2210/pdb1s3l/pdb
PDB entry 1s3l coloured by chain, front view.
PDB entry 1s3l coloured by chain, side view.
PDB entry 1s3l coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structural and functional characterization of a novel phosphodiesterase from Methanococcus jannaschii.
Chen S, Yakunin AF, Kuznetsova E, Busso D, Pufan R, Proudfoot M, Kim R, Kim SH
J Biol Chem 279 31854-62 (2004)
PMID: 15128743

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176606 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphodiesterase MJ0936 Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.92 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58346 (Residues: 1-165; Coverage: 99%)
Gene name: MJ0936
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

2 bound ligands:
Ligand UNX 2 x UNX
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2, ALS BEAMLINE 5.0.1
Spacegroup: I41
Unit cell:
a: 83.722Å b: 83.722Å c: 149.472Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.253
Expression system: Escherichia coli

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Citations

7 review citations

'Conserved hypothetical' proteins: prioritization of targets for experimental study.
Galperin et al. (2004)
6 more

2 mentions without citation

Robust recognition of zinc binding sites in proteins.
Ebert et al. (2008)
1 more