1s26 Summary


Structure of Anthrax Edema Factor-Calmodulin-alpha,beta-methyleneadenosine 5'-triphosphate Complex Reveals an Alternative Mode of ATP Binding to the Catalytic Site

The structure was published by Shen, Y., Guo, Q., Zhukovskaya, N.L., Drum, C.L., Bohm, A., and Tang, W.-J., in 2004 in a paper entitled "Structure of anthrax edema factor-calmodulin-adenosine-5'-(alpha,beta-methylene)-triphosphate complex reveals an alternative mode of ATP binding to the catalytic site" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.0 Å and deposited in 2004.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 2 biomacromolecules, namely Calmodulin-sensitive adenylate cyclase and Calmodulin.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Calmodulin-sensitive adenylate cyclase P40136 (291-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 510 98%
B Calmodulin-sensitive adenylate cyclase P40136 (291-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 510 98%
C Calmodulin-sensitive adenylate cyclase P40136 (291-800) (CYAA_BACAN)search Bacillus anthracissearch 96% 510 98%
D Calmodulin P62158 (2-149) (CALM_HUMAN)search Homo sapienssearch 97% 148 96%
E Calmodulin P62158 (2-149) (CALM_HUMAN)search Homo sapienssearch 97% 148 96%
F Calmodulin P62158 (2-149) (CALM_HUMAN)search Homo sapienssearch 97% 148 96%

This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P40136 (291 - 800) Calmodulin-sensitive adenylate cyclase Bacillus anthracis
P62158 (2 - 149) Calmodulin Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B, C (P40136) Adenylylcyclase toxin (the edema factor)search Adenylylcyclase toxin superfamilysearch PF03497: Anthrax toxin LF subunitsearch
D, E, F (P62158) Calmodulin-likesearch EF-handsearch PF13499: EF-hand domain pairsearch, PF13833: EF-hand domain pairsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B, C (P40136) calcium- and calmodulin-responsive adenylate cyclase activitysearch pathogenesissearch extracellular regionsearch
D, E, F (P62158) calcium ion bindingsearch protein bindingsearch ion channel bindingsearch protein domain specific bindingsearch protein N-terminus bindingsearch nitric-oxide synthase regulator activitysearch enzyme regulator activitysearch N-terminal myristoylation domain bindingsearch protein kinase bindingsearch phospholipase bindingsearch titin bindingsearch phosphatidylinositol 3-kinase bindingsearch metal ion bindingsearch protein phosphatase activator activitysearch thioesterase bindingsearch adenylate cyclase bindingsearch protein serine/threonine kinase activator activitysearch calcium-dependent protein bindingsearch nitric-oxide synthase bindingsearch type 3 metabotropic glutamate receptor bindingsearch platelet degranulationsearch response to corticosteronesearch positive regulation of protein serine/threonine kinase activitysearch positive regulation of cyclic nucleotide metabolic processsearch regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulumsearch positive regulation of peptidyl-threonine phosphorylationsearch positive regulation of protein autophosphorylationsearch innate immune responsesearch substantia nigra developmentsearch blood coagulationsearch activation of adenylate cyclase activitysearch positive regulation of nitric-oxide synthase activitysearch positive regulation of cyclic-nucleotide phosphodiesterase activitysearch rhodopsin mediated signaling pathwaysearch synaptic transmissionsearch regulation of nitric-oxide synthase activitysearch detection of calcium ionsearch regulation of cytokinesissearch carbohydrate metabolic processsearch response to calcium ionsearch signal transductionsearch muscle contractionsearch membrane organizationsearch positive regulation of protein dephosphorylationsearch neurotrophin TRK receptor signaling pathwaysearch regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ionsearch glycogen catabolic processsearch epidermal growth factor receptor signaling pathwaysearch nitric oxide metabolic processsearch fibroblast growth factor receptor signaling pathwaysearch inositol phosphate metabolic processsearch G-protein coupled receptor signaling pathwaysearch calcium-mediated signalingsearch glucose metabolic processsearch regulation of rhodopsin mediated signaling pathwaysearch regulation of cell communication by electrical coupling involved in cardiac conductionsearch response to amphetaminesearch regulation of cardiac muscle contractionsearch Fc-epsilon receptor signaling pathwaysearch small molecule metabolic processsearch phototransduction, visible lightsearch positive regulation of ryanodine-sensitive calcium-release channel activitysearch activation of phospholipase C activitysearch negative regulation of ryanodine-sensitive calcium-release channel activitysearch regulation of high voltage-gated calcium channel activitysearch regulation of ryanodine-sensitive calcium-release channel activitysearch positive regulation of phosphoprotein phosphatase activitysearch regulation of heart ratesearch negative regulation of peptidyl-threonine phosphorylationsearch platelet activationsearch nucleoplasmsearch cytosolsearch extracellular vesicular exosomesearch spindle microtubulesearch calcium channel complexsearch growth conesearch plasma membranesearch neuron projectionsearch cytoplasmsearch nucleussearch centrosomesearch extracellular regionsearch cytoskeletonsearch spindle polesearch vesiclesearch sarcomeresearch

Chain InterPro annotation
A, B, C Anthrax toxin, edema factor, centralsearch
D, E, F EF-hand domainsearch EF-hand domain pairsearch EF-Hand 1, calcium-binding sitesearch