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A novel NAD binding protein revealed by the crystal structure of E. Coli 2,3-diketogulonate reductase (YiaK) NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER82

Source organism: Escherichia coli
Biochemical function: NAD+ binding
Biological process: not assigned
Cellular component: cytoplasm

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EC 1.1.1.130: 3-dehydro-L-gulonate 2-dehydrogenase

Reaction catalysed:
3-dehydro-L-gulonate + NAD(P)(+) = (4R,5S)-4,5,6-trihydroxy-2,3-dioxohexanoate + NAD(P)H
Systematic name:
3-dehydro-L-gulonate:NAD(P)(+) 2-oxidoreductase
Alternative Name(s):
  • 2,3-diketo-L-gulonate reductase
  • 3-keto-L-gulonate dehydrogenase
  • 3-ketogulonate dehydrogenase

GO terms

Sequence family

Pfam Protein family (Pfam)
PF02615
Domain description: Malate/L-lactate dehydrogenase
Occurring in:
  1. 2,3-diketo-L-gulonate reductase
The deposited structure of PDB entry 1s20 contains 8 copies of Pfam domain PF02615 (Malate/L-lactate dehydrogenase) in 2,3-diketo-L-gulonate reductase. Showing 1 copy in chain G.

InterPro InterPro annotations
IPR003767
Domain description: Malate/L-lactate dehydrogenase-like
Occurring in:
  1. 2,3-diketo-L-gulonate reductase
IPR043143
Domain description: Malate/L-sulfolactate/L-lactate dehydrogenase-like, NADPH binding domain
Occurring in:
  1. 2,3-diketo-L-gulonate reductase
IPR043144
Domain description: Malate/L-sulfolactate/L-lactate dehydrogenase-like, alpha-helical domain
Occurring in:
  1. 2,3-diketo-L-gulonate reductase
IPR036111
Domain description: Malate/L-sulfolactate/L-lactate dehydrogenase-like superfamily
Occurring in:
  1. 2,3-diketo-L-gulonate reductase
IPR023689
Domain description: 2,3-diketo-L-gulonate reductase
Occurring in:
  1. 2,3-diketo-L-gulonate reductase

Structure domains

CATH CATH domains
3.30.1370.60
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Ribosomal Protein S8; Chain: A, domain 1
Homology: Hypothetical oxidoreductase yiak; domain 2
Occurring in:
  1. 2,3-diketo-L-gulonate reductase
The deposited structure of PDB entry 1s20 contains 8 copies of CATH domain 3.30.1370.60 (Ribosomal Protein S8; Chain: A, domain 1) in 2,3-diketo-L-gulonate reductase. Showing 1 copy in chain G.
1.10.1530.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Hypothetical Oxidoreductase Yiak; Chain: A, domain 1
Homology: Hypothetical Oxidoreductase Yiak; Chain: A, domain 1
Occurring in:
  1. 2,3-diketo-L-gulonate reductase
The deposited structure of PDB entry 1s20 contains 8 copies of CATH domain 1.10.1530.10 (Hypothetical Oxidoreductase Yiak; Chain: A, domain 1) in 2,3-diketo-L-gulonate reductase. Showing 1 copy in chain G.
3.30.60.50
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Wheat Germ Agglutinin (Isolectin 2); domain 1
Homology: Hypothetical oxidoreductase yiak; domain 3
Occurring in:
  1. 2,3-diketo-L-gulonate reductase
The deposited structure of PDB entry 1s20 contains 8 copies of CATH domain 3.30.60.50 (Wheat Germ Agglutinin (Isolectin 2); domain 1) in 2,3-diketo-L-gulonate reductase. Showing 1 copy in chain G.
SCOP SCOP annotation
89734
Class: Alpha and beta proteins (a/b)
Fold: L-sulfolactate dehydrogenase-like
Superfamily: L-sulfolactate dehydrogenase-like
Occurring in:
  1. 2,3-diketo-L-gulonate reductase
The deposited structure of PDB entry 1s20 contains 8 copies of SCOP domain 89734 (L-sulfolactate dehydrogenase-like) in 2,3-diketo-L-gulonate reductase. Showing 1 copy in chain G.

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