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PDBe Entry: 1s1h view

Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. This file, 1S1H, Contains 40S subunit. The 60S Ribosomal Subunit Is In File 1S1I.
Summary
Header RIBOSOMEsearch
Method ELECTRON MICROSCOPY
Experiment
Released 25/05/2004, deposition: 06/01/2004, last revision: 22/09/2009
Authors Spahn, C.M.search; Gomez-Lorenzo, M.G.search; Grassucci, R.A.search; Jorgensen, R.search; Andersen, G.R.search; Beckmann, R.search; Penczek, P.A.search; Ballesta, J.P.G.search; Frank, J.search
Primary citation Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation.
EMBO J.search vol:23, pag:1008-1019 (2004) [PubMed ID 14976550 ]search
Keywords 80S ribosomesearch, 40S ribosomal subunitsearch, eEF2search, tRNA translocationsearch, sordarinsearch, cryo-EMsearch
Organism Saccharomyces cerevisiae(baker's yeast) 4932search(A T B C D E G H I J K L M N O Q S)
UniProt Elongation factor 2 (EF-2) (Translation elongation factor 2) (Eukaryotic elongation factor 2) (eEF2) (Ribosomal translocase) P32324search (T)
40S ribosomal protein S0-A (Nucleic acid-binding protein NAB1A) P32905search (B)
40S ribosomal protein S3 (YS3) (RP13) P05750search (C)
40S ribosomal protein S9-A (S13) (YS11) (RP21) (YP28) O13516search (D)
40S ribosomal protein S2 (S4) (YS5) (RP12) (Omnipotent suppressor protein SUP44) P25443search (E)
40S ribosomal protein S5 (S2) (YS8) (RP14) P26783search (G)
40S ribosomal protein S22-A (S24) (YS22) (RP50) (YP58) P0C0W1search (H)
40S ribosomal protein S16 (RP61R) P40213search (I)
40S ribosomal protein S20 P38701search (J)
40S ribosomal protein S14-A (RP59A) P06367search (K)
40S ribosomal protein S23 (S28) (YS14) (RP37) P32827search (L)
40S ribosomal protein S18 P35271search (M)
40S ribosomal protein S29-B (S36) (YS29) P41058search (N)
40S ribosomal protein S13 (S27a) (YS15) P05756search (O)
40S ribosomal protein S11 (S18) (YS12) (RP41) P26781search (Q)
40S ribosomal protein S15 (S21) (YS21) (RP52) (RIG protein) Q01855search (S)
Related entries 1k5x, 1k5y, 1mms, 1mzp, 1ffk, 1fjf, 1fka, 1gix, 1giy, 1nou, 1s1i, 1s1h
Polymers
Id Name Type UniProt Residues Observed
A 18S ribosomal RNA RNA
1507 97%
T Elongation factor 2 Protein P32324 (EF2_YEAST)search
842 97%
B 40S ribosomal protein S0-A Protein P32905 (RSSA1_YEAST)search
185 100%
C 40S ribosomal protein S3 Protein P05750 (RS3_YEAST)search
192 97%
D 40S ribosomal protein S9-A Protein O13516 (RS9A_YEAST)search
179 99%
E 40S ribosomal protein S2 Protein P25443 (RS2_YEAST)search
149 100%
G 40S ribosomal protein S5 Protein P26783 (RS5_YEAST)search
150 100%
H 40S ribosomal protein S22 Protein P0C0W1 (RS22A_YEAST)search
129 98%
I 40S ribosomal protein S16 Protein P40213 (RS16_YEAST)search
142 97%
J 40S ribosomal protein S20 Protein P38701 (RS20_YEAST)search
100 97%
K 40S ribosomal protein S14-A Protein P06367 (RS14A_YEAST)search
136 91%
L 40S ribosomal protein S23 Protein P32827 (RS23_YEAST)search
118 100%
M 40S ribosomal protein S18 Protein P35271 (RS18_YEAST)search
132 99%
N 40S ribosomal protein S29-B Protein P41058 (RS29B_YEAST)search
36 80%
O 40S ribosomal protein S13 Protein P05756 (RS13_YEAST)search
65 100%
Q 40S ribosomal protein S11 Protein P26781 (RS11_YEAST)search
76 100%
S 40S ribosomal protein S15 Protein Q01855 (RS15_YEAST)search
80 100%
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