› 1rwg

X-ray diffraction
1.5Å resolution

Crystal structure of Arthrobacter aurescens chondroitin AC lyase in complex with chondroitin tetrasaccharide

Released:
DOI: 10.2210/pdb1rwg/pdb
PDB entry 1rwg coloured by chain, front view.
PDB entry 1rwg coloured by chain, side view.
PDB entry 1rwg coloured by chain, top view.
Source organism: Paenarthrobacter aurescens
Primary publication:
High-resolution crystal structure of Arthrobacter aurescens chondroitin AC lyase: an enzyme-substrate complex defines the catalytic mechanism.
Lunin VV, Li Y, Linhardt RJ, Miyazono H, Kyogashima M, Kaneko T, Bell AW, Cygler M
J Mol Biol 337 367-86 (2004)
PMID: 15003453

Function and Biology Details

Reaction catalysed: 
Eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160840 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):

Ligands and Environments

Carbohydrate polymer : NEW Components: ASG, GCT, GAD
Carbohydrate polymer
2 bound ligands:
Ligand NA 1 x NA
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 57.683Å b: 86.449Å c: 80.566Å
α: 90° β: 106.87° γ: 90°
R-values:
R R work R free
0.137 0.136 0.173

Quick links

Citations

10 review citations

Structural and mechanistic classification of uronic acid-containing polysaccharide lyases.
Garron et al. (2010)
9 more

3 mentions without citation

Mating in the Closest Living Relatives of Animals Is Induced by a Bacterial Chondroitinase.
Woznica et al. (2017)
2 more