› 1rwb

X-ray diffraction
2Å resolution

Cooperative Effect of Two Surface Amino Acid Mutations (Q252L and E170K) of Glucose Dehydrogenase from Bacillus megaterium IWG3 for the stabilization of Oligomeric State

Released:
DOI: 10.2210/pdb1rwb/pdb
PDB entry 1rwb coloured by chain, front view.
PDB entry 1rwb coloured by chain, side view.
PDB entry 1rwb coloured by chain, top view.
Source organism: Priestia megaterium
Primary publication:
Cooperative effect of two surface amino acid mutations (Q252L and E170K) in glucose dehydrogenase from Bacillus megaterium IWG3 on stabilization of its oligomeric state.
Baik SH, Michel F, Aghajari N, Haser R, Harayama S
Appl Environ Microbiol 71 3285-93 (2005)
PMID: 15933031

Function and Biology Details

Reaction catalysed: 
D-glucose + NAD(P)(+) = D-glucono-1,5-lactone + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose 1-dehydrogenase Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 261 amino acids
Theoretical weight: 28.1 KDa
Source organism: Priestia megaterium
Expression system: Escherichia coli
UniProt:
  • Canonical: P40288 (Residues: 1-261; Coverage: 100%)
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: C2
Unit cell:
a: 118.5Å b: 65.4Å c: 118Å
α: 90° β: 92.6° γ: 90°
R-values:
R R work R free
0.224 0.224 0.267
Expression system: Escherichia coli

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Citations

14 citation in other articles

Development of a thermostable glucose dehydrogenase by a structure-guided consensus concept.
Vázquez-Figueroa et al. (2007)
13 more

2 mentions without citation

Cooperative effect of two surface amino acid mutations (Q252L and E170K) in glucose dehydrogenase from Bacillus megaterium IWG3 on stabilization of its oligomeric state.
Baik et al. (2005)
1 more