› 1rv7

X-ray diffraction
2.7Å resolution

Crystal structures of a Multidrug-Resistant HIV-1 Protease Reveal an Expanded Active Site Cavity

Released:
DOI: 10.2210/pdb1rv7/pdb
PDB entry 1rv7 coloured by chain, front view.
PDB entry 1rv7 coloured by chain, side view.
PDB entry 1rv7 coloured by chain, top view.
Source organism: Human immunodeficiency virus 1
Primary publication:
Crystal structures of a multidrug-resistant human immunodeficiency virus type 1 protease reveal an expanded active-site cavity.
Logsdon BC, Vickrey JF, Martin P, Proteasa G, Koepke JI, Terlecky SR, Wawrzak Z, Winters MA, Merigan TC, Kovari LC
J Virol 78 3123-32 (2004)
PMID: 14990731

Function and Biology Details

Reaction catalysed: 
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase A2 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 99 amino acids
Theoretical weight: 10.74 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9QM22 (Residues: 1-99; Coverage: 100%)
Gene name: pol
Sequence domains: Retroviral aspartyl protease
Structure domains: Acid Proteases

Ligands and Environments

1 bound ligand:
Ligand AB1 1 x AB1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41
Unit cell:
a: 44.975Å b: 44.975Å c: 103.676Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.271 0.35
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Proteases and protease inhibitors in infectious diseases.
Agbowuro et al. (2018)
3 more

7 mentions without citation

Close correspondence between the motions from principal component analysis of multiple HIV-1 protease structures and elastic network modes.
Yang et al. (2008)
6 more