› 1rlc

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF THE UNACTIVATED RIBULOSE 1, 5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE COMPLEXED WITH A TRANSITION STATE ANALOG, 2-CARBOXY-D-ARABINITOL 1,5-BISPHOSPHATE

Released:
DOI: 10.2210/pdb1rlc/pdb
PDB entry 1rlc coloured by chain, front view.
PDB entry 1rlc coloured by chain, side view.
PDB entry 1rlc coloured by chain, top view.
Source organism: Nicotiana tabacum
Primary publication:
Crystal structure of the unactivated ribulose 1,5-bisphosphate carboxylase/oxygenase complexed with a transition state analog, 2-carboxy-D-arabinitol 1,5-bisphosphate.
Zhang KY, Cascio D, Eisenberg D
Protein Sci 3 64-9 (1994)
PMID: 8142899

Function and Biology Details

Reaction catalysed: 
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexadecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133653 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribulose bisphosphate carboxylase large chain Chain: L
Molecule details ›
Chain: L
Length: 477 amino acids
Theoretical weight: 52.97 KDa
Source organism: Nicotiana tabacum
Expression system: Not provided
UniProt:
  • Canonical: P00876 (Residues: 1-477; Coverage: 100%)
Gene names: rbcA, rbcL
Sequence domains:
Structure domains:
Ribulose bisphosphate carboxylase small subunit, chloroplastic Chain: S
Molecule details ›
Chain: S
Length: 123 amino acids
Theoretical weight: 14.57 KDa
Source organism: Nicotiana tabacum
Expression system: Not provided
UniProt:
  • Canonical: P69249 (Residues: 58-180; Coverage: 68%)
Gene name: RBCS
Sequence domains: Ribulose bisphosphate carboxylase, small chain
Structure domains: Ribulose bisphosphate carboxylase, small subunit

Ligands and Environments

1 bound ligand:
Ligand CAP 1 x CAP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I422
Unit cell:
a: 149Å b: 149Å c: 136.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 not available
Expression system: Not provided

Quick links

Citations

3 review citations

Structure and function of Rubisco.
Andersson et al. (2008)
2 more

3 mentions without citation

"Hidden" sequence periodicities and protein architecture.
Rackovsky S. (1998)
2 more