› 1rjz

X-ray diffraction
2.6Å resolution

Mhc Class I Natural Mutant H-2Kbm8 Heavy Chain Complexed With beta-2 Microglobulin and Herpies Simplex Virus Mutant Glycoprotein B Peptide

Released:
DOI: 10.2210/pdb1rjz/pdb
PDB entry 1rjz coloured by chain, front view.
PDB entry 1rjz coloured by chain, side view.
PDB entry 1rjz coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the restoration of TCR recognition of an MHC allelic variant by peptide secondary anchor substitution.
Miley MJ, Messaoudi I, Metzner BM, Wu Y, Nikolich-Zugich J, Fremont DH
J Exp Med 200 1445-54 (2004)
PMID: 15557346

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135043 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 280 amino acids
Theoretical weight: 32.18 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01901 (Residues: 22-301; Coverage: 81%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Envelope glycoprotein B Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 8 amino acids
Theoretical weight: 965 Da
Source organism: Human alphaherpesvirus 1 strain F
Expression system: Not provided
UniProt:
  • Canonical: P06436 (Residues: 498-505; Coverage: 1%)
Gene names: UL27, gB

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 66.572Å b: 90.177Å c: 88.984Å
α: 90° β: 111.32° γ: 90°
R-values:
R R work R free
0.23 0.23 0.288
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Low-affinity peptides and T-cell selection.
Ziegler et al. (2009)
2 more

1 mention without citation

Structural basis for the restoration of TCR recognition of an MHC allelic variant by peptide secondary anchor substitution.
Miley et al. (2004)