› 1rcq

X-ray diffraction
1.45Å resolution

The 1.45 A crystal structure of alanine racemase from a pathogenic bacterium, Pseudomonas aeruginosa, contains both internal and external aldimine forms

Released:
DOI: 10.2210/pdb1rcq/pdb
PDB entry 1rcq coloured by chain, front view.
PDB entry 1rcq coloured by chain, side view.
PDB entry 1rcq coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure at 1.45 A resolution of alanine racemase from a pathogenic bacterium, Pseudomonas aeruginosa, contains both internal and external aldimine forms.
LeMagueres P, Im H, Dvorak A, Strych U, Benedik M, Krause KL
Biochemistry 42 14752-61 (2003)
PMID: 14674749

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190936 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase, catabolic Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 39.16 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9HTQ2 (Residues: 1-357; Coverage: 100%)
Gene names: PA5302, dadX
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand DLY 1 x DLY
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: C2221
Unit cell:
a: 72.679Å b: 76.129Å c: 136.266Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.149 0.206
Expression system: Escherichia coli BL21

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Citations

3 review citations

Structure, function and dynamics in the mur family of bacterial cell wall ligases.
Smith CA. (2006)
2 more

10 mentions without citation

Residues Asp164 and Glu165 at the substrate entryway function potently in substrate orientation of alanine racemase from E. coli: Enzymatic characterization with crystal structure analysis.
Wu et al. (2008)
9 more