› 1r9m

X-ray diffraction
2.1Å resolution

Crystal Structure of Human Dipeptidyl Peptidase IV at 2.1 Ang. Resolution.

Released:
DOI: 10.2210/pdb1r9m/pdb
PDB entry 1r9m coloured by chain, front view.
PDB entry 1r9m coloured by chain, side view.
PDB entry 1r9m coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human dipeptidyl peptidase IV in complex with a decapeptide reveals details on substrate specificity and tetrahedral intermediate formation.
Aertgeerts K, Ye S, Tennant MG, Kraus ML, Rogers J, Sang BC, Skene RJ, Webb DR, Prasad GS
Protein Sci 13 412-21 (2004)
PMID: 14718659

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Dipeptidyl peptidase 4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 733 amino acids
Theoretical weight: 85.15 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P27487 (Residues: 39-766; Coverage: 95%)
Gene names: ADCP2, CD26, DPP4
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 21 x NAG
Ligand FUC 1 x FUC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 121.823Å b: 124.056Å c: 144.491Å
α: 90° β: 114.71° γ: 90°
R-values:
R R work R free
0.219 0.218 0.249
Expression system: Trichoplusia ni

Quick links

Citations

13 review citations

An emerging role of dipeptidyl peptidase 4 (DPP4) beyond glucose control: potential implications in cardiovascular disease.
Zhong et al. (2013)
12 more

6 mentions without citation

DPP-4 inhibitors for treating T2DM - hype or hope? an analysis based on the current literature.
Saini et al. (2023)
5 more