› 1r8e

X-ray diffraction
2.4Å resolution

Crystal Structure of BmrR Bound to DNA at 2.4A Resolution

Released:
DOI: 10.2210/pdb1r8e/pdb
PDB entry 1r8e coloured by chain, front view.
PDB entry 1r8e coloured by chain, side view.
PDB entry 1r8e coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The structural mechanism for transcription activation by MerR family member multidrug transporter activation, N terminus.
Newberry KJ, Brennan RG
J Biol Chem 279 20356-62 (2004)
PMID: 14985361

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114504 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Multidrug-efflux transporter 1 regulator Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 32.62 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P39075 (Residues: 1-278; Coverage: 100%)
Gene names: BSU24020, bmr1R, bmrR
Sequence domains:
Structure domains:
5'-D(*GP*AP*CP*CP*CP*TP*CP*CP*CP*CP*TP*TP*AP*GP*GP*GP*GP*AP*GP*GP*GP*TP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 23 nucleotides
Theoretical weight: 7.06 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand GOL 5 x GOL
Ligand P4P 1 x P4P
Ligand IMD 11 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P4322
Unit cell:
a: 107.28Å b: 107.28Å c: 145.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.267
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

Activating transcription in bacteria.
Lee et al. (2012)
16 more

9 mentions without citation

Diversity of structure and function of response regulator output domains.
Galperin MY. (2010)
8 more