› 1r1p

X-ray diffraction
1.8Å resolution

Structural Basis for Differential Recognition of Tyrosine Phosphorylated Sites in the Linker for Activation of T cells (LAT) by the Adaptor Protein Gads

Released:
DOI: 10.2210/pdb1r1p/pdb
PDB entry 1r1p coloured by chain, front view.
PDB entry 1r1p coloured by chain, side view.
PDB entry 1r1p coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for differential recognition of tyrosine-phosphorylated sites in the linker for activation of T cells (LAT) by the adaptor Gads.
Cho S, Velikovsky CA, Swaminathan CP, Houtman JC, Samelson LE, Mariuzza RA
EMBO J 23 1441-51 (2004)
PMID: 15029250

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131608 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GRB2-related adaptor protein 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 100 amino acids
Theoretical weight: 11.82 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O89100 (Residues: 50-147; Coverage: 30%)
Gene names: Gads, Grap2, Grb2l, Grid, Mona
Sequence domains: SH2 domain
Structure domains: SH2 domain
LAT pY171 peptide Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 7 amino acids
Theoretical weight: 830 Da
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 10 x SO4
1 modified residue:
Ligand PTR 4 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 90.307Å b: 90.307Å c: 145.959Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.241
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Experimental detection of short regulatory motifs in eukaryotic proteins: tips for good practice as well as for bad.
Gibson et al. (2015)
3 more

3 mentions without citation

Bridging the Gap: Modulatory Roles of the Grb2-Family Adaptor, Gads, in Cellular and Allergic Immune Responses.
Yablonski D. (2019)
2 more