› 1qzh

X-ray diffraction
2.4Å resolution

Crystal structure of Pot1 (protection of telomere)- ssDNA complex

Released:
DOI: 10.2210/pdb1qzh/pdb
PDB entry 1qzh coloured by chain, front view.
PDB entry 1qzh coloured by chain, side view.
PDB entry 1qzh coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA.
Lei M, Podell ER, Baumann P, Cech TR
Nature 426 198-203 (2003)
PMID: 14614509

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114481 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Protection of telomeres protein 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 187 amino acids
Theoretical weight: 21.28 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O13988 (Residues: 2-185; Coverage: 33%)
Gene names: SPAC26H5.06, pot1
Sequence domains: Telomeric single stranded DNA binding POT1/CDC13
Structure domains: Nucleic acid-binding proteins
telomeric single-stranded DNA Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 6 nucleotides
Theoretical weight: 1.82 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 131.614Å b: 76.329Å c: 140.13Å
α: 90° β: 115.21° γ: 90°
R-values:
R R work R free
0.249 0.249 0.283
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

27 review citations

Human telomere, oncogenic promoter and 5'-UTR G-quadruplexes: diverse higher order DNA and RNA targets for cancer therapeutics.
Patel et al. (2007)
26 more

7 mentions without citation

Single-stranded DNA-binding proteins: multiple domains for multiple functions.
Dickey et al. (2013)
6 more