› 1qz9

X-ray diffraction
1.85Å resolution

The Three Dimensional Structure of Kynureninase from Pseudomonas fluorescens

Released:
DOI: 10.2210/pdb1qz9/pdb
PDB entry 1qz9 coloured by chain, front view.
PDB entry 1qz9 coloured by chain, side view.
PDB entry 1qz9 coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Three-dimensional structure of kynureninase from Pseudomonas fluorescens.
Momany C, Levdikov V, Blagova L, Lima S, Phillips RS
Biochemistry 43 1193-203 (2004)
PMID: 14756555

Function and Biology Details

Reaction catalysed: 
L-kynurenine + H(2)O = anthranilate + L-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160742 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kynureninase Chain: A
Molecule details ›
Chain: A
Length: 416 amino acids
Theoretical weight: 45.95 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P83788 (Residues: 1-416; Coverage: 100%)
Gene name: kynU
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
2 bound ligands:
Ligand CL 1 x CL
Ligand P3G 1 x P3G
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 68.076Å b: 68.076Å c: 137.976Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.153 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Nicotinamide adenine dinucleotide metabolism as an attractive target for drug discovery.
Khan et al. (2007)
3 more

6 mentions without citation

Crystal structure of Homo sapiens kynureninase.
Lima et al. (2007)
5 more