1qwn Summary


GOLGI ALPHA-MANNOSIDASE II Covalent Intermediate Complex with 5-fluoro-gulosyl-fluoride

The structure was published by Numao, S., Kuntz, D.A., Withers, S.G., and Rose, D.R., in 2003 in a paper entitled "Insights into the mechanism of Drosophila melanogaster Golgi alpha-mannosidase II through the structural analysis of covalent reaction intermediates." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 1.2 Å and deposited in 2003.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of Alpha-mannosidase II. This molecule has the UniProt identifier Q24451 (MAN2_DROME)search. The sample contained 1045 residues which is 93% of the natural sequence. Out of 1045 residues 1014 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule is most likely monomeric.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A Alpha-mannosidase II Q24451 (76-1108) (MAN2_DROME)search Drosophila melanogastersearch 92% 1045 97%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
Q24451 (76 - 1108) Alpha-mannosidase II Drosophila melanogaster

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (Q24451) alpha-mannosidase, domain 2search, alpha-mannosidase, C-terminal domainsearch, alpha-mannosidasesearch Glycoside hydrolase 38, N terminal domainsearch, Families 57/38 glycoside transferase; middle domainsearch, Golgi alpha-mannosidase IIsearch, Golgi alpha-mannosidase II; domain 4search, Immunoglobulin-likesearch PF01074: Glycosyl hydrolases family 38 N-terminal domainsearch, PF07748: Glycosyl hydrolases family 38 C-terminal domainsearch, PF09261: Alpha mannosidase, middle domainsearch

Chain ID Cellular component (GO) Molecular function (GO) Biological process (GO)
A (Q24451) Golgi stacksearch membranesearch endoplasmic reticulumsearch integral component of membranesearch Golgi apparatussearch Golgi membranesearch mannosidase activitysearch hydrolase activitysearch catalytic activitysearch alpha-mannosidase activitysearch carbohydrate bindingsearch hydrolase activity, hydrolyzing O-glycosyl compoundssearch zinc ion bindingsearch metal ion bindingsearch mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase activitysearch hydrolase activity, acting on glycosyl bondssearch metabolic processsearch mannose metabolic processsearch carbohydrate metabolic processsearch encapsulation of foreign targetsearch protein glycosylationsearch protein deglycosylationsearch

Chain InterPro annotation
A Glycoside hydrolase family 38, N-terminal domainsearch Galactose mutarotase-like domainsearch Glycoside hydrolase/deacetylase, beta/alpha-barrelsearch Glycosyl hydrolase family 38, C-terminalsearch Glycosyl hydrolase, family 13, all-betasearch Glycoside hydrolase, family 38, central domainsearch Glycoside hydrolase 38/57, N-terminal domainsearch Glycoside hydrolase, families 57/38, central domainsearch