› 1qva

X-ray diffraction
2.5Å resolution

YEAST INITIATION FACTOR 4A N-TERMINAL DOMAIN

Released:
DOI: 10.2210/pdb1qva/pdb
PDB entry 1qva coloured by chain, front view.
PDB entry 1qva coloured by chain, side view.
PDB entry 1qva coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystallographic structure of the amino terminal domain of yeast initiation factor 4A, a representative DEAD-box RNA helicase.
Johnson ER, McKay DB
RNA 5 1526-34 (1999)
PMID: 10606264

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase eIF4A Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 24.95 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P10081 (Residues: 2-224; Coverage: 57%)
Gene names: J0660, TIF1, TIF2, TIF41A, TIF41B, YJL138C, YKR059W
Sequence domains: DEAD/DEAH box helicase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P212121
Unit cell:
a: 38.8Å b: 74.3Å c: 81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.223 0.274
Expression system: Escherichia coli

Quick links

Citations

11 review citations

The DEAD-box protein family of RNA helicases.
Cordin et al. (2006)
10 more

7 mentions without citation

Crystal structure of Mycobacterium tuberculosis SecA, a preprotein translocating ATPase.
Sharma et al. (2003)
6 more