› 1qrk

X-ray diffraction
2.5Å resolution

HUMAN FACTOR XIII WITH STRONTIUM BOUND IN THE ION SITE

Released:
DOI: 10.2210/pdb1qrk/pdb
PDB entry 1qrk coloured by chain, front view.
PDB entry 1qrk coloured by chain, side view.
PDB entry 1qrk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of the calcium binding site and a novel ytterbium site in blood coagulation factor XIII by x-ray crystallography.
Fox BA, Yee VC, Pedersen LC, Le Trong I, Bishop PD, Stenkamp RE, Teller DC
J Biol Chem 274 4917-23 (1999)
PMID: 9988734

Function and Biology Details

Reaction catalysed: 
A protein-L-glutamine + a protein-L-lysine = a protein with an N(6)-(gamma-glutamyl)-L-lysine cross-link + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132554 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coagulation factor XIII A chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 731 amino acids
Theoretical weight: 83.23 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00488 (Residues: 2-732; Coverage: 100%)
Gene names: F13A, F13A1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SR 2 x SR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 101.9Å b: 72.32Å c: 135.03Å
α: 90° β: 105.9° γ: 90°
R-values:
R R work R free
0.183 0.183 0.275
Expression system: Saccharomyces cerevisiae

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Citations

16 review citations

Transglutaminases: crosslinking enzymes with pleiotropic functions.
Lorand et al. (2003)
15 more

1 mention without citation

Structure functional insights into calcium binding during the activation of coagulation factor XIII A.
Singh et al. (2019)