› 1qpi

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF TETRACYCLINE REPRESSOR/OPERATOR COMPLEX

Released:
DOI: 10.2210/pdb1qpi/pdb
PDB entry 1qpi coloured by chain, front view.
PDB entry 1qpi coloured by chain, side view.
PDB entry 1qpi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system.
Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W
Nat Struct Biol 7 215-9 (2000)
PMID: 10700280

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114458 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Tetracycline repressor protein class D Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 22.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACT4 (Residues: 4-206; Coverage: 93%)
Gene name: tetR
Sequence domains:
Structure domains:
DNA (5'-D(*CP*CP*TP*AP*TP*CP*AP*AP*TP*GP*AP*TP*AP*GP*A)-3') Chain: M
Molecule details ›
Chain: M
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(*TP*CP*TP*AP*TP*CP*AP*TP*TP*GP*AP*TP*AP*GP*G)-3') Chain: N
Molecule details ›
Chain: N
Length: 15 nucleotides
Theoretical weight: 4.6 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: C2221
Unit cell:
a: 38.04Å b: 193.2Å c: 91.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.307
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

40 review citations

The TetR family of transcriptional repressors.
Ramos et al. (2005)
39 more

34 mentions without citation

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
33 more