› 1qno

X-ray diffraction
2Å resolution

The 3-D structure of a Trichoderma reesei b-mannanase from glycoside hydrolase family 5

Released:
DOI: 10.2210/pdb1qno/pdb
PDB entry 1qno coloured by chain, front view.
PDB entry 1qno coloured by chain, side view.
PDB entry 1qno coloured by chain, top view.
Source organism: Trichoderma reesei
Primary publication:
The three-dimensional structure of a Trichoderma reesei beta-mannanase from glycoside hydrolase family 5.
Sabini E, Schubert H, Murshudov G, Wilson KS, Siika-Aho M, Penttilä M
Acta Crystallogr D Biol Crystallogr 56 3-13 (2000)
PMID: 10666621

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189172 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mannan endo-1,4-beta-mannosidase A Chain: A
Molecule details ›
Chain: A
Length: 344 amino acids
Theoretical weight: 37.75 KDa
Source organism: Trichoderma reesei
Expression system: Trichoderma reesei
UniProt:
  • Canonical: Q99036 (Residues: 28-371; Coverage: 82%)
Gene name: man1
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand NAG 4 x NAG
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 50.01Å b: 54.27Å c: 60.24Å
α: 90° β: 111.31° γ: 90°
R-values:
R R work R free
0.126 0.126 0.2
Expression system: Trichoderma reesei

Quick links

Citations

3 review citations

Microbial mannanases: an overview of production and applications.
Dhawan et al. (2007)
2 more

13 mentions without citation

Structural and biochemical analyses of glycoside hydrolase families 5 and 26 β-(1,4)-mannanases from Podospora anserina reveal differences upon manno-oligosaccharide catalysis.
Couturier et al. (2013)
12 more