› 1qhg

X-ray diffraction
2.5Å resolution

STRUCTURE OF DNA HELICASE MUTANT WITH ADPNP

Released:
DOI: 10.2210/pdb1qhg/pdb
PDB entry 1qhg coloured by chain, front view.
PDB entry 1qhg coloured by chain, side view.
PDB entry 1qhg coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
DNA binding mediates conformational changes and metal ion coordination in the active site of PcrA helicase.
Soultanas P, Dillingham MS, Velankar SS, Wigley DB
J Mol Biol 290 137-48 (1999)
PMID: 10388562

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent DNA helicase PcrA Chain: A
Molecule details ›
Chain: A
Length: 724 amino acids
Theoretical weight: 82.6 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P56255 (Residues: 1-724; Coverage: 100%)
Gene name: pcrA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS
Spacegroup: P65
Unit cell:
a: 138.098Å b: 138.098Å c: 111.086Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.22 0.269
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structure and mechanism of helicases and nucleic acid translocases.
Singleton et al. (2007)
11 more

3 mentions without citation

Taking a molecular motor for a spin: helicase mechanism studied by spin labeling and PELDOR.
Constantinescu-Aruxandei et al. (2016)
2 more