› 1qgn

X-ray diffraction
2.9Å resolution

CYSTATHIONINE GAMMA-SYNTHASE FROM NICOTIANA TABACUM

Released:
DOI: 10.2210/pdb1qgn/pdb
PDB entry 1qgn coloured by chain, front view.
PDB entry 1qgn coloured by chain, side view.
PDB entry 1qgn coloured by chain, top view.
Source organism: Nicotiana tabacum
Primary publication:
The crystal structure of cystathionine gamma-synthase from Nicotiana tabacum reveals its substrate and reaction specificity.
Steegborn C, Messerschmidt A, Laber B, Streber W, Huber R, Clausen T
J Mol Biol 290 983-96 (1999)
PMID: 10438597

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195699 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine gamma-synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 445 amino acids
Theoretical weight: 48.07 KDa
Source organism: Nicotiana tabacum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9ZPL5 (Residues: 1-445; Coverage: 100%)
Gene name: metB
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PLP 8 x PLP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 120Å b: 129.5Å c: 309.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.25
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Synthesis of the sulfur amino acids: cysteine and methionine.
Wirtz et al. (2005)
1 more

7 mentions without citation

Evolutionarily conserved regions and hydrophobic contacts at the superfamily level: The case of the fold-type I, pyridoxal-5'-phosphate-dependent enzymes.
Paiardini et al. (2004)
6 more