› 1qff

X-ray diffraction
2.7Å resolution

E. COLI FERRIC HYDROXAMATE UPTAKE RECEPTOR (FHUA) IN COMPLEX WITH BOUND FERRICHROME-IRON

Released:
DOI: 10.2210/pdb1qff/pdb
PDB entry 1qff coloured by chain, front view.
PDB entry 1qff coloured by chain, side view.
PDB entry 1qff coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
A conserved structural motif for lipopolysaccharide recognition by procaryotic and eucaryotic proteins.
Ferguson AD, Welte W, Hofmann E, Lindner B, Holst O, Coulton JW, Diederichs K
Structure 8 585-92 (2000)
PMID: 10873859

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Ferrichrome outer membrane transporter/phage receptor Chain: A
Molecule details ›
Chain: A
Length: 725 amino acids
Theoretical weight: 80.52 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P06971 (Residues: 34-438, 439-747; Coverage: 100%)
Gene names: JW0146, b0150, fhuA, tonA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: PA1, GCN, KDO, GMH, GLC, GLA
Carbohydrate polymer
8 bound ligands:
Ligand PO4 2 x PO4
Ligand FTT 4 x FTT
Ligand DAO 1 x DAO
Ligand MYR 1 x MYR
Ligand DPO 1 x DPO
Ligand EAP 1 x EAP
Ligand FCI 1 x FCI
Ligand DDQ 2 x DDQ
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P61
Unit cell:
a: 171.4Å b: 171.4Å c: 85.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.231 0.276
Expression system: Escherichia coli

Quick links

Citations

27 review citations

Lipopolysaccharide endotoxins.
Raetz et al. (2002)
26 more

13 mentions without citation

TonB-dependent transporters: regulation, structure, and function.
Noinaj et al. (2010)
12 more