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EBI PDBe PDBeView

PDBe Entry: 1q81 view

Crystal Structure of minihelix with 3' puromycin bound to A-site of the 50S ribosomal subunit.
Summary
Header RIBOSOMEsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 2.95 Å, R-factor: 21.0%, Free R-factor: 25.9%, Spacegroup: C 2 2 21
Released 07/10/2003, deposition: 20/08/2003, last revision: 24/02/2009
Authors Hansen, J.L.search; Schmeing, T.M.search; Moore, P.B.search; Steitz, T.A.search
Primary citation Structural Insights into Peptide Bond Formation
PROC.NATL.ACAD.SCI.USAsearch vol:99, pag:11670-11675 (2002) [PubMed ID 12185246 ]search
Keywords ribosome 50Ssearch, substrate analogsearch, minihelixsearch, A-sitesearch, PROTEIN-PROTEIN COMPLEXsearch, RNA-RNA COMPLEXsearch, PROTEIN-RNA COMPLEXsearch
Organism Haloarcula marismortui 2238search(A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 1 2 3 4)
UniProt 50S ribosomal protein L2P (Hmal2) (Hl4) P20276search (C)
50S ribosomal protein L3P (Hmal3) (Hl1) P20279search (D)
50S ribosomal protein L4P (Hmal4) (Hl6) P12735search (E)
50S ribosomal protein L5P (Hmal5) (Hl13) P14124search (F)
50S ribosomal protein L6P (Hmal6) (Hl10) P14135search (G)
50S ribosomal protein L7Ae (Hs6) P12743search (H)
50S ribosomal protein L10E (Ribosomal protein L10) (Acidic ribosomal protein P0 homolog) (L10E) (HMal10) P15825search (I)
50S ribosomal protein L10e P60617search (J)
50S ribosomal protein L13P (Hmal13) P29198search (K)
50S ribosomal protein L14P (Hmal14) (Hl27) P22450search (L)
50S ribosomal protein L15P (Hmal15) (Hl9) P12737search (M)
50S ribosomal protein L15e (50S ribosomal protein LC12) P60618search (N)
50S ribosomal protein L18P (Hmal18) (Hl12) P14123search (O)
50S ribosomal protein L18e (Hl29) (L19) P12733search (P)
50S ribosomal protein L19e (Hmal19) (Hl24) P14119search (Q)
50S ribosomal protein L21e (Hl31) P12734search (R)
50S ribosomal protein L22P (Hmal22) (Hl23) P10970search (S)
50S ribosomal protein L23P (Hmal23) (Hl25) (L21) P12732search (T)
50S ribosomal protein L24P (Hmal24) (Hl16) (Hl15) P10972search (U)
50S ribosomal protein L24e (Hl21/Hl22) P14116search (V)
50S ribosomal protein L29P (Hmal29) (Hl33) P10971search (W)
50S ribosomal protein L30P (Hmal30) (Hl20) (Hl16) P14121search (X)
50S ribosomal protein L31e (L34) (Hl30) P18138search (Y)
50S ribosomal protein L32e (Hl5) P12736search (Z)
50S ribosomal protein L37Ae P60619search (1)
50S ribosomal protein L37e (L35e) P32410search (2)
50S ribosomal protein L39e (Hl39e) (Hl46e) P22452search (3)
50S ribosomal protein L44E (La) (Hla) P32411search (4)
Solvent 1, 2, 3, 4, A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z
Related entries 1fgo, 1ffz, 1m90, 1kqs, 1q82, 1q86, 1q7y
Polymers
Id Name Type UniProt Residues Observed
A 23S ribosomal rna RNA
2922 94%
B 5S ribosomal RNA RNA
122 100%
5 minihelix-puromycin RNA
2 100%
C 50S ribosomal protein L2P Protein P20276 (RL2_HALMA)search
 239 99%
D 50S ribosomal protein L3P Protein P20279 (RL3_HALMA)search
 337 100%
E 50S ribosomal protein L4E Protein P12735 (RL4_HALMA)search
 246 100%
F 50S ribosomal protein L5P Protein P14124 (RL5_HALMA)search
 176 79%
G 50S ribosomal protein L6P Protein P14135 (RL6_HALMA)search
 177 97%
H 50S ribosomal protein L7Ae Protein P12743 (RL7A_HALMA)search
 119 100%
I Acidic ribosomal protein P0 homolog Protein P15825 (RLA0_HALMA)search
 348 8%
J L10 Ribosomal Protein Protein P60617 (RL10_HALMA)search
 167 93%
K 50S ribosomal protein L13P Protein P29198 (RL13_HALMA)search
 145 97%
L 50S ribosomal protein L14P Protein P22450 (RL14_HALMA)search
 132 100%
M 50S ribosomal protein L15P Protein P12737 (RL15_HALMA)search
 164 88%
N L15 Ribosomal Protein Protein P60618 (RL15E_HALMA)search
 194 100%
O 50S ribosomal protein L18P Protein P14123 (RL18_HALMA)search
 186 100%
P 50S ribosomal protein L18e Protein P12733 (RL18E_HALMA)search
 115 100%
Q 50S ribosomal protein L19E Protein P14119 (RL19_HALMA)search
 148 96%
R 50S ribosomal protein L21e Protein P12734 (RL21_HALMA)search
 95 100%
S 50S ribosomal protein L22P Protein P10970 (RL22_HALMA)search
 154 97%
T 50S ribosomal protein L23P Protein P12732 (RL23_HALMA)search
 84 96%
U 50S ribosomal protein L24P Protein P10972 (RL24_HALMA)search
 119 100%
V 50S ribosomal protein L24E Protein P14116 (RL24E_HALMA)search
 66 80%
W 50S ribosomal protein L29P Protein P10971 (RL29_HALMA)search
 70 92%
X 50S ribosomal protein L30P Protein P14121 (RL30_HALMA)search
 154 100%
Y 50S ribosomal protein L31e Protein P18138 (RL31_HALMA)search
 91 90%
Z 50S ribosomal protein L32E Protein P12736 (RL32_HALMA)search
 240 59%
1 L37Ae 50S ribosomal protein Protein P60619 (RL37A_HALMA)search
 73 100%
2 50S ribosomal protein L37e Protein P32410 (RL37_HALMA)search
 56 100%
3 50S ribosomal protein L39e Protein P22452 (RL39_HALMA)search
 48 95%
4 50S ribosomal protein L44E Protein P32411 (RL44_HALMA)search
 92 100%
Heterogens
Id Name Ligands
A, B, C, D, L, U, Z, 1, 4 MAGNESIUM ION MG search
A POTASSIUM ION K search
A, B, C, E, J, K, M, N, R, S, T SODIUM ION NA search
A, C, D, K, N, O, P, S, Z, 4 CHLORIDE ION CL search
5 PUROMYCIN-5'-MONOPHOSPHATE PPU search
P, V, 1, 2, 4 CADMIUM ION CD search
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