› 1q6z

X-ray diffraction
1Å resolution

HIGH RESOLUTION STRUCTURE OF E28A MUTANT BENZOYLFORMATE DECARBOXYLASE FROM PSEUDOMONAS PUTIDA COMPLEXED WITH THIAMIN THIAZOLONE DIPHOSPHATE

Released:
DOI: 10.2210/pdb1q6z/pdb
PDB entry 1q6z coloured by chain, front view.
PDB entry 1q6z coloured by chain, side view.
PDB entry 1q6z coloured by chain, top view.
Source organism: Pseudomonas putida
Entry authors: Bera AK, Hasson MS

Function and Biology Details

Reaction catalysed: 
Benzoylformate = benzaldehyde + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Benzoylformate decarboxylase Chain: A
Molecule details ›
Chain: A
Length: 528 amino acids
Theoretical weight: 56.34 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P20906 (Residues: 1-528; Coverage: 100%)
Gene name: mdlC
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TZD 1 x TZD
2 bound ligands:
Ligand MG 1 x MG
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: I222
Unit cell:
a: 81.371Å b: 95.6Å c: 136.881Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.122 0.122 0.14
Expression system: Escherichia coli

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