› 1q6x

X-ray diffraction
2.5Å resolution

Crystal structure of rat choline acetyltransferase

Released:
DOI: 10.2210/pdb1q6x/pdb
PDB entry 1q6x coloured by chain, front view.
PDB entry 1q6x coloured by chain, side view.
PDB entry 1q6x coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Choline acetyltransferase structure reveals distribution of mutations that cause motor disorders.
Cai Y, Cronin CN, Engel AG, Ohno K, Hersh LB, Rodgers DW
EMBO J 23 2047-58 (2004)
PMID: 15131697

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + choline = CoA + O-acetylcholine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Choline O-acetyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 644 amino acids
Theoretical weight: 72.48 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32738 (Residues: 1-636; Coverage: 99%)
Gene name: Chat
Sequence domains: Choline/Carnitine o-acyltransferase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 84.701Å b: 78.882Å c: 139.364Å
α: 90° β: 98.38° γ: 90°
R-values:
R R work R free
0.226 0.223 0.251
Expression system: Escherichia coli

Quick links

Citations

6 review citations

What have we learned from the congenital myasthenic syndromes.
Engel et al. (2010)
5 more

5 mentions without citation

Averaged kick maps: less noise, more signal... and probably less bias.
Pražnikar et al. (2009)
4 more