1q3n Summary


Crystal structure of KDO8P synthase in its binary complex with substrate PEP

The structure was published by Vainer, R., Belakhov, V., Rabkin, E., Baasov, T., and Adir, N., in 2005 in a paper entitled "Crystal structures of Escherichia coli KDO8P synthase complexes reveal the source of catalytic irreversibility." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.7 Å and deposited in 2003.

The experimental data on which the structure is based was also deposited.

The PDB entry contains the structure of 2-dehydro-3-deoxyphosphooctonate aldolase. This molecule has the UniProt identifier P0A715 (KDSA_ECOLI)search. The sample contained 284 residues which is 100% of the natural sequence. Out of 284 residues 272 were observed and are deposited in the PDB.

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms homotetramers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A 2-dehydro-3-deoxyphosphooctonate aldolase P0A715 (1-284) (KDSA_ECOLI)search Escherichia coli K-12search 100% 284 95%

This entry contains 1 unique UniProt protein:

UniProt accession Name Organism PDB
P0A715 (1 - 284) 2-dehydro-3-deoxyphosphooctonate aldolase Escherichia coli

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A (P0A715) Class I DAHP synthetasesearch Aldolase class Isearch PF00793: DAHP synthetase I familysearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A (P0A715) biosynthetic processsearch metabolic processsearch keto-3-deoxy-D-manno-octulosonic acid biosynthetic processsearch lipopolysaccharide biosynthetic processsearch catalytic activitysearch 3-deoxy-8-phosphooctulonate synthase activitysearch transferase activitysearch cytoplasmsearch cytosolsearch

Chain InterPro annotation
A DAHP synthetase I/KDSAsearch 3-deoxy-8-phosphooctulonate synthasesearch Aldolase-type TIM barrelsearch