› 1q18

X-ray diffraction
2.36Å resolution

Crystal structure of E.coli glucokinase (Glk)

Released:
DOI: 10.2210/pdb1q18/pdb
PDB entry 1q18 coloured by chain, front view.
PDB entry 1q18 coloured by chain, side view.
PDB entry 1q18 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of Escherichia coli ATP-dependent glucokinase and its complex with glucose.
Lunin VV, Li Y, Schrag JD, Iannuzzi P, Cygler M, Matte A
J Bacteriol 186 6915-27 (2004)
PMID: 15466045

Function and Biology Details

Reaction catalysed: 
ATP + D-glucose = ADP + D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 332 amino acids
Theoretical weight: 36.25 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6V9 (Residues: 2-321; Coverage: 100%)
Gene names: ECs3268, Z3654, glk
Sequence domains: Glucokinase
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P43212
Unit cell:
a: 81.474Å b: 81.474Å c: 234.71Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.267
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
5 more

11 mentions without citation

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Olenginski et al. (2021)
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